Blue-white capabl
Phage T1 resistant
End A -/RecA
Restriction-deficient (RMS)
Clone methylated DNA
Inducible plasmid copy number
F’episome
Antibiotic resistance
Electrocompetent:efficiency
Chemically competent:efficiency
常规克隆及文库构建
E. cloni® 10G (Classic)
√
√
√
√
Ø
Ø
Str
≥5 x109
106-109
E. cloni 10G (Elite)
≥2 x1010
-
E. cloni 10G (Supreme)
≥4 x1010
E.cloni 10G F’（Elite)
E. cloni 5-alpha
Ø
-
-
≥1 x108
不稳定的插入物或有毒基因
Copycutter™ EPI400™
√
≥1 x1010
≥1 x107
大片段,plasmids和fosmids质粒
TransforMax™ EC100™
Ø
≥5 x108
TransforMax EPI300™
a
TransforMax EPI300™-T1R
BAC克隆
BAC-Optimised Replicator™ v2.0
Amp, Str
E. cloni BAC-Optimised 10G
BigEasy™-TSA
b
c
噬菌体展示
TG1
Ø
d
ER2738
Tet
SS320 (MC1061 F’)
e
Tet, Str
MC1061 F-
CRISPR sgRNA文库及慢病毒质粒克隆
Endura™
f
g
Site-directed mutagenesis
CJ236
Cam
≥1 x109
Amp, ampicillin; Cam, chloramphenicol; Str,streptomycin; Tet,tetracycline a:Requires OriV vectors (e.g., CopyRight® andpCCFOS)b: RequiresIPTGc: Only with pJAZZ®vectorsd: mcrB- only; notsuitable for cloning eukaryotic genomicDNAe: mcrA- mcrB-; notsuitable for cloning eukaryotic genomicDNAf: RecA13onlyg: mcrB- mrr-; notsuitable for cloning eukaryotic genomicDNA
Primarypromoter
Background expressioncontrol
Lowendotoxin
Antibioticresistance
Electrocompetent:efficiency
Chemicallycompetent:efficiency
常规蛋白表达
E. cloni EXPRESSBL21(DE3)
T7
Ø
Ø
-
≥5 x 109
≥1 x 107
Hl-Control™BL21(DE3)
T7
√
Gen
-
Hl-Control™10G
Plac,Ptac,Ptrc,T5lac,rhaPBAD
Gen, Str
≥1 x 109
ClearColi®BL21(DE3)
T7
√
≥1 x 109
-
带毒性蛋白表达
OverExpress™C41(DE3)
≥1 × 109
≥1 × 106
OverExpress C41(DE3)pLysS
√
Cam
OverExpressC43(DE3)
OverExpress C43(DE3)pLysS