lucigen特色酶制剂产品列表

北京中北林格供应Biosearch technologies lucigen特色酶制剂产品列表，中北林格是Biosearchtechnologies lucigen总代理，提供Biosearch technologieslucigen产品货号报价查询。代理的其他品牌包括Lucigen总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。

目前Biosearch technologies lucigen特色酶制剂产品列表可查询货号为352个，种类非常齐全。

公司前身是成立于1990年的专业从事动物血清生产及销售的生物技术公司，自有品牌zhongbeilinge™产品鼠血清远销欧美，为美国、加拿大、德国的一些欧美公司以及国内知名学府提供了优质产品和良好服务。2005年，公司在德国布莱梅新增设了服务于欧洲市场的代理商。

在国内，销售网络已铺就各个中心城市，做到了产品齐全、价格合理、经营稳健、信息反馈及时，并能随时随地享受国外供应商直接、周到、完美的售后服务。

以下是Biosearch technologies lucigen特色酶制剂产品列表详情：

特色酶制剂

分子生物学用

Mesophilic DNA polymerases

Product name

Activity

5′→3′  exonuclease

3′→5′ exonuclease

Optimum temp.

Heat inactivationa

Strand displacement

Bst DNA Polymerase, Exonuclease Minus

-

-

55-65 °C

80 °C for 20 minutes

++++

NxGen phi29 DNA Polymerase

++

30 °C

65 °C for 10 minutes

+++++

Exo-Minus Klenow DNA Polymerase (D355A, E357A)

37 °C

n.d.

+

a Indicated treatment results incomplete inactivation under standard reaction conditions; n.d., notdetermined.

Thermophilic DNA polymerases

Product name

Activity

5′→3′  exonuclease

Optimum temp.

Thermostabilitya

Fidelityb

EconoTaq DNA Polymerase

+

70-72 °C

n.d.

n.d.

MasterAmp Taq DNA Polymerase

10 minutes at 97 °C

0.38-1.82 x104

MasterAmp Tth DNA Polymerase

68-74 °C

2.2 x 104

LavaLAMP® DNA Enzyme

n.d.

LavaLAMP® RNA Enzyme

a Values represent half-lives: 50% of theenzymatic activity is retained after the given time at the statedtemperature.

bDefined as the average number of correctnucleotides a polymerase incorporates before making an error; n.d.,not determined.

RNA polymerases

Product name

Activity

5′→3′  exonuclease

3′→5′ exonuclease

Optimum temp.

Heat inactivation

NxGen T7 RNA Polymerase

-

-

37 °C

n.d.

T7 R&DNA Polymerase

Poly(A) Polymerase

not recommended

n.d., not determined.

Reverse transcriptases

Enzyme

Activity

Substrates

RNase H activity

Optimum temp.

Heat inactivation

MMLV High Performance Reverse Transcriptase

Synthesises first-strand cDNA

ssRNA, ssDNA

+

37 °C

85 °C for 5 minutes

NxGen M-MuLV Reverse Transcriptase

37-42 °C

85 °C for 10 minutes

EpiScript RNase H~ Reverse Transcriptase

-

Enzyme properties

Enzymes for molecular biology

DNA endonucleases

Enzyme

Substrate

Activity

Products

Applications

Optimum temp.

Heat inactivation

Baseline-ZERO DNase

dsDNA and ssDNA

Digests dsDNA or ssDNA to mononucleotides. In presence ofMg2+, it cleaves each DNA strand of dsDNArandomly and independently

Mononucleotides

Removing DNA from RNA preparations

37 °C

65 °C for 10 minutesa

RNase-Free DNase  I

Activated by divalent cations. In presence ofMg2+, it cleaves each DNA strand of dsDNArandomly and independently, preferentially adjacent to pyrimidines.In presence of Mn2+, it cleaves bothstrands simultaneously, generating fragments with blunt ends or 1 -to 2-base overhangs.

Oligos and dNMPs with 5′P and3′OH

•  Removing DNA from RNA preparations •   Random nicking of dsDNA •    DNasefootprinting

a In the presence of the provided StopSolution,

n.d., not determined.

DNA exonucleases

Enzyme

Substrate

Activity

Products

Applications

Optimum temp.

Heat inactivation

Exonuclease I (E. coli)

ssDNA

3′→5′ exonucleasethat digests ssDNA in the presence ofMg2+_

dNMPs

Removal of ssDNA and oligonucleotides.

80 °C for 15 minutes

Exonuclease III (E. coli)

dsDNA

3′→5′ exonucleasethat digests duplex DNA from the 3′end ofa nick, or a blunt or 3′-recessed end;not active on thionucleotides. Exo III also has RNase H,3′-DNA phosphatase, and apurinic DNAendonuclease activities.

dNMPs and ssDNA on the opposite strand. Partial digestionproduces dsDNA having 5' extensions of ssDNA.

•    Used with S1 Nucle-ase or Mung BeanNuclease to make nested deletions •    Preparationof ssDNA templates for sequencing •    Site-directedmutagenesis •    Preparation of labeledstrand-specific probes

65 °C for 10 minutes

Exonuclease VII

Exonuclease that digests ssDNA in both5′→3′ and3′→ 5′ directions.

Removal of primers and single-stranded oligos.

n.d.

Plasmid-Safe ATP-Dependent DNase

linear ssDNA and dsDNA

Selectively digests linear DNA. No activity on nicked orclosed-circular dsDNA.

Removal of chromosomal DNA fragments from plasmid, fosmid,and BAC preparations.

70 °C for 30 minutes

Rec J Exonuclease

5′→3′ exonucleasethat digests ssDNA in the presence ofMg2+_

Removal of primers and ssDNA from dsDNA.

65 °C for 20 minutes

Nucleases active on both DNA and RNA

Enzyme

Activity

Products

Applications

Heat inactivation

Terminator5′-Phosphate-Dependent Exonuclease

ssDNA or ssRNA

5′→3′ exonucleasethat digests ssDNA or ssRNA with5′-monophosphorylated ends, but not with5′-OH,5′-triphosphorylated, or5′-capped ends

dNMPs or NMPs

•    Removal of5′-monophosphorylat-ed DNA or primers oroligos •    Enrichment of ssDNA or ssRNA moleculeslacking 5′-monophos-phategroups

30 °C (Buffer A) 42 °C (Buffer B)

not recommended

OmniCleave Endonuclease

ssDNA, dsDNA, or RNA

Endonuclease that efficiently digests DNA andRNA

di-, tri-, and tetra-nucleotides

•    Removal of DNA and RNA from proteinpreparations •    Removal of host DNA from phagepreparations.

25-37 °C

RNA nucleases

Enzyme

Substrate

Activity

Products

Applications

Optimum temp.

RNase A

ssRNA

Cleaves ssRNA 3′ ofpyrimidine residues.

Oligoribonucleo-tides with 3'-cyti-dine or 3'-uridineresidues

•    Removal of RNA from DNA preparations •   RNase protection assays •    RNAmapping and structure studies

37 °C (15-70 °C)

RNase 1, E. coli

Cleaves ssRNA between all dinucleotide pairs.

NMPs with 5'-OH and 2'，3’-cyclicmonophosphate

•    Removal of RNA from DNA preparations •   RNase protection assays •   Mismatch detection of single basepairs inRNA:RNAor RNA:DNA hybrids

37 °C

70 °C for 20 minutes (in presence of 5 mM DTT)

Hybridase Thermostable RNase H

RNA in RNA:DNA hybrid

Cleaves RNA in RNA:DNA hybrid without affectingunhybridised RNA or DNA.

Oligoribonucleo-tides with 5' phosphate and 3'OH.

High-stringency hybrid selection.

45-70 °C

RNase R

linear RNA

Digests linear RNA, including the ssRNA end of lariatstructures, but not circular RNA or dsRNA with3′ overhangs <7 nt.

Oligoribonucleo-tides with 5’ phosphate and 3'OH

•Alternative splicing and gene expression studies •   Intron cDNA production •   Intronic screening of cDNA libraries

n.d.

n.d., not determined.

Enzyme properties

Enzymes for molecular biology

Ligases

Name of ligase

Cofactor

Ligation template required to ligate

Type of ends ligated

Primary application

Optimum temp.

Heat inactivation

Blunt

Cohesive

NxGen T4 DNA Ligase

ATP

Noa

Yes

Yes

Cloning

4-25 °C

70 °C for 15 minutes

Ampligase Thermostable DNA Ligase

NAD

Yes; DNA only

No

Template-dependent ligation

45-65 °C

not recommended

Fast-Link DNA Ligase

Rapid cloning

16-25°C

70 °C for 15 minutes

T4 RNA Ligase 2, Deletion Mutant

Not needed

No

Ligates ss adenylated DNA or RNA to small RNAs

Ligation of RNA to RNA

37 °C

n.d.

CircLigase ssDNA Ligase

Self-ligates (circularises) ssDNA or ssRNA with 5' P and3' OH

Making ssDNA circles for rolling-circle replication,transcription, and small-RNA sequencing

60 °C

80 °C for 10 minutes

CircLigase II ssDNA Ligase

a. These enzymes ligate blunt ends of dsDNA, but ligationis more efficient with cohesive ends,

n.d., not determined.

Phosphatases and kinases

Enzyme

Substrate

Activity

Products

Applications

Heat inactivation

RNA5' Polyphosphatase

5'-di or tri-phosphorylated RNA

Removes y and β  phosphates

5'-mono- phosphorylated RNA

•    Ligation-tagging •   Analysis of 5’-end structure

T4 Polynucleotide Kinase, Cloned

DNA, RNA

Catalyses transfer of Y phosphate of ATP to 5’ terminus ofDNA (ds/ss) or RNA (with 3' OH)

Phosphorylated DNA, RNA

Addition of 5' phosphate to DNA or RNA

70 °C for 5 minutes

n.d., not determined.

RNA-guided endonucleases

Enzyme

Modifications

Concentration

PAM preference

Type of edit

Guide RNA length

CRISPRcraft S.p. Cas9 Nuclease

One C-terminal NLS, one C-terminal 6 x His tag

10mg/mL(62 µM)

G-rich (NGG)

Blunt double-stranded break

〜97nt

AsCpf1 Nuclease

Two C-terminal NLS, one C-terminal 6 x His tag

10 mg/mL (64 µM)

T-rich (TTTV)

Staggered double-stranded break

〜41 nt

NLS, nuclear localisation signal; PAM,protospacer-adjacent motif.