北京中北林格供应Biosearch technologies lucigen特色酶制剂产品列表，中北林格是Biosearchtechnologies lucigen总代理，提供Biosearch technologieslucigen产品货号报价查询。代理的其他品牌包括Lucigen总代理，epicentre总代理，illumina总代理，ICLLAB总代理，ImmunoReagents总代理。
目前Biosearch technologies lucigen特色酶制剂产品列表可查询货号为352个，种类非常齐全。
公司前身是成立于1990年的专业从事动物血清生产及销售的生物技术公司，自有品牌zhongbeilinge™产品鼠血清远销欧美，为美国、加拿大、德国的一些欧美公司以及国内知名学府提供了优质产品和良好服务。2005年，公司在德国布莱梅新增设了服务于欧洲市场的代理商。
在国内，销售网络已铺就各个中心城市，做到了产品齐全、价格合理、经营稳健、信息反馈及时，并能随时随地享受国外供应商直接、周到、完美的售后服务。
以下是Biosearch technologies lucigen特色酶制剂产品列表详情：
特色酶制剂
分子生物学用
Mesophilic DNA polymerases
Product name
Activity
5′→3′ exonuclease
3′→5′ exonuclease
Optimum temp.
Heat inactivationa
Strand displacement
Bst DNA Polymerase, Exonuclease Minus
-
-
55-65 °C
80 °C for 20 minutes
++++
NxGen phi29 DNA Polymerase
++
30 °C
65 °C for 10 minutes
+++++
Exo-Minus Klenow DNA Polymerase (D355A, E357A)
37 °C
n.d.
+
a Indicated treatment results incomplete inactivation under standard reaction conditions; n.d., notdetermined.
Thermophilic DNA polymerases
Product name
Activity
5′→3′ exonuclease
Optimum temp.
Thermostabilitya
Fidelityb
EconoTaq DNA Polymerase
+
70-72 °C
n.d.
n.d.
MasterAmp Taq DNA Polymerase
10 minutes at 97 °C
0.38-1.82 x104
MasterAmp Tth DNA Polymerase
68-74 °C
2.2 x 104
LavaLAMP® DNA Enzyme
n.d.
LavaLAMP® RNA Enzyme
a Values represent half-lives: 50% of theenzymatic activity is retained after the given time at the statedtemperature.
bDefined as the average number of correctnucleotides a polymerase incorporates before making an error; n.d.,not determined.
RNA polymerases
Product name
Activity
5′→3′ exonuclease
3′→5′ exonuclease
Optimum temp.
Heat inactivation
NxGen T7 RNA Polymerase
-
-
37 °C
n.d.
T7 R&DNA Polymerase
Poly(A) Polymerase
not recommended
n.d., not determined.
Reverse transcriptases
Enzyme
Activity
Substrates
RNase H activity
Optimum temp.
Heat inactivation
MMLV High Performance Reverse Transcriptase
Synthesises first-strand cDNA
ssRNA, ssDNA
+
37 °C
85 °C for 5 minutes
NxGen M-MuLV Reverse Transcriptase
37-42 °C
85 °C for 10 minutes
EpiScript RNase H~ Reverse Transcriptase
-
Enzyme properties
Enzymes for molecular biology
DNA endonucleases
Enzyme
Substrate
Activity
Products
Applications
Optimum temp.
Heat inactivation
Baseline-ZERO DNase
dsDNA and ssDNA
Digests dsDNA or ssDNA to mononucleotides. In presence ofMg2+, it cleaves each DNA strand of dsDNArandomly and independently
Mononucleotides
Removing DNA from RNA preparations
37 °C
65 °C for 10 minutesa
RNase-Free DNase I
Activated by divalent cations. In presence ofMg2+, it cleaves each DNA strand of dsDNArandomly and independently, preferentially adjacent to pyrimidines.In presence of Mn2+, it cleaves bothstrands simultaneously, generating fragments with blunt ends or 1 -to 2-base overhangs.
Oligos and dNMPs with 5′P and3′OH
• Removing DNA from RNA preparations • Random nicking of dsDNA • DNasefootprinting
a In the presence of the provided StopSolution,
n.d., not determined.
DNA exonucleases
Enzyme
Substrate
Activity
Products
Applications
Optimum temp.
Heat inactivation
Exonuclease I (E. coli)
ssDNA
3′→5′ exonucleasethat digests ssDNA in the presence ofMg2+_
dNMPs
Removal of ssDNA and oligonucleotides.
80 °C for 15 minutes
Exonuclease III (E. coli)
dsDNA
3′→5′ exonucleasethat digests duplex DNA from the 3′end ofa nick, or a blunt or 3′-recessed end;not active on thionucleotides. Exo III also has RNase H,3′-DNA phosphatase, and apurinic DNAendonuclease activities.
dNMPs and ssDNA on the opposite strand. Partial digestionproduces dsDNA having 5' extensions of ssDNA.
• Used with S1 Nucle-ase or Mung BeanNuclease to make nested deletions • Preparationof ssDNA templates for sequencing • Site-directedmutagenesis • Preparation of labeledstrand-specific probes
65 °C for 10 minutes
Exonuclease VII
Exonuclease that digests ssDNA in both5′→3′ and3′→ 5′ directions.
Removal of primers and single-stranded oligos.
n.d.
Plasmid-Safe ATP-Dependent DNase
linear ssDNA and dsDNA
Selectively digests linear DNA. No activity on nicked orclosed-circular dsDNA.
Removal of chromosomal DNA fragments from plasmid, fosmid,and BAC preparations.
70 °C for 30 minutes
Rec J Exonuclease
5′→3′ exonucleasethat digests ssDNA in the presence ofMg2+_
Removal of primers and ssDNA from dsDNA.
65 °C for 20 minutes
Nucleases active on both DNA and RNA
Enzyme
Activity
Products
Applications
Heat inactivation
Terminator5′-Phosphate-Dependent Exonuclease
ssDNA or ssRNA
5′→3′ exonucleasethat digests ssDNA or ssRNA with5′-monophosphorylated ends, but not with5′-OH,5′-triphosphorylated, or5′-capped ends
dNMPs or NMPs
• Removal of5′-monophosphorylat-ed DNA or primers oroligos • Enrichment of ssDNA or ssRNA moleculeslacking 5′-monophos-phategroups
30 °C (Buffer A) 42 °C (Buffer B)
not recommended
OmniCleave Endonuclease
ssDNA, dsDNA, or RNA
Endonuclease that efficiently digests DNA andRNA
di-, tri-, and tetra-nucleotides
• Removal of DNA and RNA from proteinpreparations • Removal of host DNA from phagepreparations.
25-37 °C
RNA nucleases
Enzyme
Substrate
Activity
Products
Applications
Optimum temp.
RNase A
ssRNA
Cleaves ssRNA 3′ ofpyrimidine residues.
Oligoribonucleo-tides with 3'-cyti-dine or 3'-uridineresidues
• Removal of RNA from DNA preparations • RNase protection assays • RNAmapping and structure studies
37 °C (15-70 °C)
RNase 1, E. coli
Cleaves ssRNA between all dinucleotide pairs.
NMPs with 5'-OH and 2'，3’-cyclicmonophosphate
• Removal of RNA from DNA preparations • RNase protection assays • Mismatch detection of single basepairs inRNA:RNAor RNA:DNA hybrids
37 °C
70 °C for 20 minutes (in presence of 5 mM DTT)
Hybridase Thermostable RNase H
RNA in RNA:DNA hybrid
Cleaves RNA in RNA:DNA hybrid without affectingunhybridised RNA or DNA.
Oligoribonucleo-tides with 5' phosphate and 3'OH.
High-stringency hybrid selection.
45-70 °C
RNase R
linear RNA
Digests linear RNA, including the ssRNA end of lariatstructures, but not circular RNA or dsRNA with3′ overhangs <7 nt.
Oligoribonucleo-tides with 5’ phosphate and 3'OH
•Alternative splicing and gene expression studies • Intron cDNA production • Intronic screening of cDNA libraries
n.d.
n.d., not determined.
Enzyme properties
Enzymes for molecular biology
Ligases
Name of ligase
Cofactor
Ligation template required to ligate
Type of ends ligated
Primary application
Optimum temp.
Heat inactivation
Blunt
Cohesive
NxGen T4 DNA Ligase
ATP
Noa
Yes
Yes
Cloning
4-25 °C
70 °C for 15 minutes
Ampligase Thermostable DNA Ligase
NAD
Yes; DNA only
No
Template-dependent ligation
45-65 °C
not recommended
Fast-Link DNA Ligase
Rapid cloning
16-25°C
70 °C for 15 minutes
T4 RNA Ligase 2, Deletion Mutant
Not needed
No
Ligates ss adenylated DNA or RNA to small RNAs
Ligation of RNA to RNA
37 °C
n.d.
CircLigase ssDNA Ligase
Self-ligates (circularises) ssDNA or ssRNA with 5' P and3' OH
Making ssDNA circles for rolling-circle replication,transcription, and small-RNA sequencing
60 °C
80 °C for 10 minutes
CircLigase II ssDNA Ligase
a. These enzymes ligate blunt ends of dsDNA, but ligationis more efficient with cohesive ends,
n.d., not determined.
Phosphatases and kinases
Enzyme
Substrate
Activity
Products
Applications
Heat inactivation
RNA5' Polyphosphatase
5'-di or tri-phosphorylated RNA
Removes y and β phosphates
5'-mono- phosphorylated RNA
• Ligation-tagging • Analysis of 5’-end structure
T4 Polynucleotide Kinase, Cloned
DNA, RNA
Catalyses transfer of Y phosphate of ATP to 5’ terminus ofDNA (ds/ss) or RNA (with 3' OH)
Phosphorylated DNA, RNA
Addition of 5' phosphate to DNA or RNA
70 °C for 5 minutes
n.d., not determined.
RNA-guided endonucleases
Enzyme
Modifications
Concentration
PAM preference
Type of edit
Guide RNA length
CRISPRcraft S.p. Cas9 Nuclease
One C-terminal NLS, one C-terminal 6 x His tag
10mg/mL(62 µM)
G-rich (NGG)
Blunt double-stranded break
〜97nt
AsCpf1 Nuclease
Two C-terminal NLS, one C-terminal 6 x His tag
10 mg/mL (64 µM)
T-rich (TTTV)
Staggered double-stranded break
〜41 nt
NLS, nuclear localisation signal; PAM,protospacer-adjacent motif.