粪便DNA提取试剂盒

货号：116570200 品牌 MP Biomedicals  

从人类和动物的粪便样品中有效分离基因组DNA，直接用于PCR

1.多达500mg的粪便样品

2.内含配合FastPrep仪 器使用的理解介质E

3.通常可以从500mg的粪便样品中分离得到10-20μg总DNA

4.去除对下游实验有影响的

快速高效提取粪便DNA，可裂解任何粪便样品。可直接用于PCR等其下游实验，TheFastDNA® SPIN Kit for Feces Designed for use with the FastPrep®Instruments, host cells as well as bacteria, fungi, viruses,protists and other cells present in fecal samples are easily lysedwithin 40 seconds. These benchtop devices use a unique, optimizedmotion that provide an extremely quick, efficient and highlyreproducible homogenization that surpasses traditional extractionmethods.

Samples are placed into 2.0 mL tubes containing Lysing MatrixE, a mixture of ceramic and silica particles designed toefficiently lyse all stool organisms. Stool samples are pre-treatedby washing in a Pre-Lysis Buffer. Homogenization takes place in thepresence of MT Buffer and Sodium Phosphate Buffer. The pre-lysingand lysing reagents were carefully developed to protect andsolubilize nucleic acids upon cell lysis, facilitate extraction ofgenomic DNA , and minimize RNA, humic acid or polyphenolcontamination. Following lysis, samples are centrifuged to pelletdebris and lysing matrix. DNA is purified from the supernatant witha silica-based GENECLEAN® procedure using SPIN filters. Eluted DNAis ready for digestion, electrophoresis, PCR and any other desiredapplication.

产品应用，如下：

The Fast DNA® SPIN Kit for Feces quickly and efficientlyisolates PCR-ready genomic DNA from fresh or frozen human andanimal stool samples.

产品属性，如下：

Product Type

Kits & Assays

Components

Lysing Matrix E 50x     2.0 mL tubes, PLSSolution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100    mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL,Wash   Buffer #2 12   mL, TES 20 mL, BBS Gel Loading Dye200 μl, SPIN Filter Tubes   50 each, Catch Tubes50 each

Instrument     Compatibility

FastPrep-24; FastPrep     120; FastPrep-96;SuperFastPrep-1

Sample Type

Soil; Feces;     Environmental Water;Wastewater; Sludge

Sample Volume

up to 500 mg per prep

Tube Compatibility

2 mL

Matrix Type

E

Molecule Enriched

DNA

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