The UV Cleavable Biotin-Azide allows the tagging ofalkyne modified substrates via copper-catalyzed clickchemistry.
Highlights:
Biotin conjugate can be subse visualized by streptavidinWestern techniques and/or enriched using streptavidin purificationmethods
Captured target can be released under mild photolysis conditions(365 nm) and results in a significant decrease of background signaldue to non-specifically bound proteins
Also available: 13C6-UV-Biotin-azide
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反应原理示意图：
参考文献：
1. Alkylation Damage by Lipid Electrophiles Targets FunctionalProtein Systems. Codreanu, S. G.; Ullery, J. C.; Zhu, J.; Tallman,K. A.; Beavers, W. N.; Porter, N. A.; Marnett, L. J.; Zhang, B.;Liebler, D. C. Mol. Cell. Proteomics 2014, 13, 849.
2. An Azido-Biotin Reagent for Use in the Isolation of ProteinAdducts of Lipid-Derived Electrophiles by Streptavidin Catch andPhoto-Release. Kim, H.-Y. H.; Tallman, K. A.; Liebler, D. C.;Porter, N. A. Mol. Cell. Proteomics 2009, 8, 2080.
3. Yang J, Gupta V, Tallman KA, Porter NA, Carroll KS, Liebler DC.Global, in situ, site-specific analysis of protein S-sulfenylation.Nat Protoc. 2015 Jul;10(7):1022-37. View Article
4. Fu L, Liu K, Ferreira RB, Carroll KS, Yang J. Proteome-WideAnalysis of Cysteine S-Sulfenylation Using a Benzothiazine-BasedProbe. Curr Protoc Protein Sci. 2019 Feb;95(1):e76. View Article